Changes between Version 3 and Version 4 of cypress/WGSA
- Timestamp:
- 07/15/20 00:07:48 (4 years ago)
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cypress/WGSA
v3 v4 1 1 [[PageOutline]] 2 2 = Installing and Setup WGSA in a local directory on Cypress = 3 This instruction is based on [https://sites.google.com/site/jpopgen/wgsa/setting-up-wgsa-linux this] page and adapted for Cypress. 4 3 5 Decide a folder dedicated for the pipeline, for example '/lustre/project/group/WGSA'. 4 6 … … 17 19 18 20 {{{ 19 mkdir $WGSA_DIR/annovar 21 mkdir $WGSA_DIR/annovar2019Oct24 20 22 }}} 21 23 22 24 23 25 Download the ANNOVAR main package from [http://download.openbioinformatics.org/annovar_download_form.php here]. 24 The package comes as annovar.latest.tar.gz, save it to $WGSA_DIR/annovar . Unzip it.26 The package comes as annovar.latest.tar.gz, save it to $WGSA_DIR/annovar2019Oct24. Unzip it. 25 27 26 28 27 29 {{{ 28 cd $WGSA_DIR/annovar 30 cd $WGSA_DIR/annovar2019Oct24 29 31 tar -zxvf annovar.latest.tar.gz 30 32 }}} 31 33 32 Download !RefSeq and !Ensembl gene models for ANNOVAR:34 Download !RefSeq and Ensembl gene models for ANNOVAR: 33 35 34 36 {{{ 35 cd $WGSA_DIR/annovar /annovar37 cd $WGSA_DIR/annovar2019Oct24/annovar 36 38 perl annotate_variation.pl -buildver hg19 -downdb -webfrom annovar refGene humandb/ 37 39 perl annotate_variation.pl -buildver hg19 -downdb -webfrom annovar ensGene humandb/ … … 57 59 58 60 {{{ 59 idev 61 idev -c 1 -t 4 60 62 }}} 61 63 It will take more than one hour. 62 64 See [https://wiki.hpc.tulane.edu/trac/wiki/Workshops/IntroToHpc2015/using#SubmittingInteractiveJobs here] for more about 'idev'. 63 65