= Run Cellranger-ARC on Cypress = See [https://support.10xgenomics.com/single-cell-multiome-atac-gex/software/pipelines/latest/using/mkfastq Generating FASTQs with cellranger-arc mkfastq]. This page to show how to run Cellranger-ARC with [https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/advanced/cluster-mode the cluster mode] on Cypress. Example SLURM script: {{{ #!/bin/bash #SBATCH --qos=normal # Quality of Service #SBATCH --job-name=mkFASTQ # Job Name #SBATCH --time=24:00:00 # WallTime #SBATCH --nodes=1 # Number of Nodes = 1 #SBATCH --ntasks-per-node=1 # Number of tasks (MPI presseces) = 1 #SBATCH --cpus-per-task=1 # Number of processors per task OpenMP threads() = 1 #SBATCH --gres=mic:0 # Number of Co-Processors #SBATCH --export=ALL #SBATCH --mail-type=ALL #SBATCH --mail-user='USERID'@tulane.edu # SET YOUR EMAIL ADDRESS module load cellranger-arc/1.0.1 cellranger-arc mkfastq --id=tiny-bcl-atac \ --run=./cellranger-arc-tiny-bcl-atac-1.0.0 \ --csv=./cellranger-arc-tiny-bcl-atac-simple-1.0.0.csv \ --jobmode=slurm \ --localmem=120 \ --localcores=20 \ --maxjobs=4 \ --jobinterval=1000 }}} With this script, 'cellranger-arc' submits at most 4 sub-jobs. You can set the maximum number of jobs by changing: {{{ --maxjobs=18 \ }}}